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PublicationYear
  
PublicationType
  
  
PublicationCommittee
  
  
  
  
  
PublicationECORegion
  
PublicationSpecies
  
Abstract
Keywords
  
  
  
ICES Book
  
Advice Statement
ICES Ecoregions
  
  
  
  
  
  
  
  
  
  
  
  
  
  
TIMES01.pdf
  
1987TimesCadium and lead: Determination in organic matrices with electrothermal furnace atomic absorption spectrophotometry
1
4/20/2021 2:15 PMRuth Anderson
A methodological approach to reduce deterioration of analyte response is the application of chemical separation techniques prior to the determination step. Extraction of metal ions in the form of a chelate by an organic solvent is one of the most suitable methods.
atomic absorption; spectrophotometry; lead; cadmium10.17895/ices.pub.5027N/A
TextU. Harms2707-6997978-87-7482-255-44/8/2019 2:40 PM
TIMES02.pdf
  
1987TimesTrace metals in sea water: Sampling and storage methods
2
4/20/2021 2:15 PMRuth Anderson
Sampling procedures for dissolved trace metals in sea water have progressed to the extent that it is now possible to describe reliable methods for the collection, preservation, and storage of seawater samples. The accumulated experience of a number of workers in the field as well as the results from several intercalibration exercises run by ICES, IOC, and others have been used to determine the best sampling procedures. For many metals a single procedure is adequate, however, for some others separate procedures must be used.
sample handling; sample storage; trace metals; seawater10.17895/ices.pub.5028N/A
TextP. A. Yeats2707-6997978-87-7482-257-84/9/2019 11:44 AM
TIMES03.pdf
  
1987TimesCadium in marine sediments: Determination by graphite furnace atomic absorption spectroscopy
3
4/20/2021 2:15 PMRuth Anderson
Cadmium is one of the most important toxic elements to be determined in environmental samples. Cd has proved, however, to be a difficult element to determine with good precision and relative accuracy. This is shown by the results of recent intercalibration exercises. It is suggested that the poor results are mostly due to the instrumental methods and inadequate use of reference materials to ensure good relative accuracy of the results. This paper discusses the various parameters that affect the precision and relative accuracy of Cd determinations and describes a simple, straightforward method based on the teflon bomb decomposition of the sample with HF-aquaregia followed by a graphite furnace atomic absorption determination of Cd using anun coated L'vovplat-form. The relative accuracy and precision of the method has been confirmed and found to be good by analyses of reference materials and through participation in intercalibration exercises.
atomic absorption; spectroscopy; graphite furnace; marine sediment; cadmium10.17895/ices.pub.5029N/A
TextR. T. T. Rantala; D. H. Loring2707-6997978-87-7482-258-54/9/2019 11:52 AM
TIMES04.pdf
  
1987TimesLipophilic organic material: An apparatus for extracting solids used for their concentration from sea water
4
4/20/2021 2:15 PMRuth Anderson
Lipophilic organic substances, whether man-made, mobilized by human activities, or of recent natural origin, are usually dissolved in sea water at such minute concentrations that the chemical characterization and quantitative determination of single compounds are possible only after a sufficient quantity has been collected by concentration from relatively large (of the order of 100-1000 litres) volumes. Essentially two techniques have found widespread application in marine organic chemistry and pollution research, i.e., extraction, either batchwise or continuous, with a suitable water-immiscible solvent or sorption onto solids. Described below is a new apparatus and technique for purifying sorbant material from substances interfering with ultra-trace analyses and for desorbing analytes.
extraction; lipophilic substances; seawater10.17895/ices.pub.5030N/A
TextM. Ehrhardt2707-6997978-87-7482-259-24/9/2019 11:55 AM
TIMES05.pdf
  
1987TimesPrimary production: Guidelines for measurement by 14C incorporation
5
4/20/2021 2:15 PMRuth Anderson
These guidelines aim to standardize, in the ICES area, the methology of primary production measurements made by determination of C incorporation, taking into account, as far as possible, the diversity of techniques in use at the time of writing. As well as dealing with purely technical aspects of the method, new concepts have been incorporated which represent considerable modifications to the current approach to primary production determination and which may alter the calculated daily production estimates.
carbon 14 incorporation; primary production measurements; methodology; standardization10.17895/ices.pub.5031N/A
TextK. Richardson2707-6997978-87-7482-260-84/9/2019 11:56 AM
TIMES06.pdf
  
1987TimesControl procedures: Good laboratory practice and quality assurance
6
4/20/2021 2:15 PMRuth Anderson
Good Laboratory Practice (GLP) is one of the manifestations of the increased attention being paid to quality control measures in general. It provides a framework designed to bring the quality of laboratory results into accord with predefined standards and to maintain the quality at this level. The need for such a framework arises from the economic, political, and scientific implications that laboratory studies may have, which place demands on the reliability and comparability of the results.
This overview provides a brief introduction to GLP and Quality Asurance. The aim of this paper is to give insight into the available literature on these topics.The several components of GLP and Quality Assurance are reviewed but not discussed in detail.
good laboratory practice (GLP); quality assurance; Quality control10.17895/ices.pub.5032N/A
TextF. A. J. M. Vijverberg; W. P. Cofino2707-6997978-87-7482-261-54/9/2019 11:58 AM
TIMES07.pdf
  
1990TimesSuspended particulate matter: Collection methods for gravimetric and trace metal analysis
7
4/20/2021 2:15 PMRuth Anderson
Filtration is the most commonly used method for separating the dissolved and particulate fractions of seawater samples. A wide variety of filter types with different pore sizes has been used for this purpose. Several studies have shown that the concentrations of suspended particulate matter (SPM) measured using different types of filters can vary significantly. This document covers guidance for the collection of SPM for gravimetric and trace metal analysis.
suspended particulate matter; gravimetric analysis; trace metals; collection methods10.17895/ices.pub.5033N/A
TextP. A. Yeats; L. Brügmann2707-6997978-87-7482-262-24/9/2019 11:59 AM
TIMES08.pdf
  
1990TimesSoft bottom macrofauna: Collection and treatment of samples
8
4/20/2021 2:15 PMRuth Anderson
The aim of these recommendations is to standardize the methods used by different scientists for long-term benthos surveys, in order to increase the comparability of results for different areas and to enable, inter alia, detection of large-scale changes in the  system that would not otherwise be detected by a single scientist. In these recommendations, soft bottoms are defined as those with sediments ranging from mud to, and including, sand.
macrofauna; soft bottom; sample handling; sample treatment; methodology; standardization10.17895/ices.pub.5034N/A
TextH. Rumohr2707-6997978-87-7482-263-94/9/2019 12:00 PM
TIMES09.pdf
  
1990TimesSediments and suspended particulate matter: Total and partial methods of digestion
9
4/20/2021 2:15 PMRuth Anderson
In order to determine the major and trace metal concentrations of marine sediments and suspended particulate matter by wet chemical methods, it is necessary to dissolve all or part of the sample. Sample digestion methods commonly used are: (a) total decomposition, (b) strong acid digestion, or (c) moderate or weak acid extractions. This leaflet describes in detail the wet chemical methods for both
total decomposition and weak acid extraction of sediments and suspended particulate matter.
marine sediment; suspended particulate matter; wet chemical methods; acid digestion; decomposition10.17895/ices.pub.5035N/A
TextD. H. Loring; R. T. T. Rantala2707-6997978-87-7482-264-64/9/2019 12:02 PM
TIMES10.pdf
  
1990TimesOrganic halogens: Determination in marine media of adsorbable, volatile, or extractable compound totals
10
4/20/2021 2:15 PMRuth Anderson
The environmental impact of the release of halogenated organic compounds to the sea has become of increasing interest during recent decades. Most compounds in this group are xenobiotic of origin and, consequently, of potential environmental hazard. Thec haracterization of domestic and industrial discharges with respect to their contribution of haloorganic compounds to the sea, as well as monitoring to determine the occurrence and levels of halo-organic compounds in the marine environment, have been major topics. This leaflet reviews frequently employed methods for the determination of organic halogens as group parameters in water samples. The methods are evaluated with respect to their applicability in wastewater characterization and marine monitoring. Results from the analysis of organic halogens in samples of marine water, sediments, and biological organisms are summarized. It appears that determinations of extractable organic halogens (EOX) in marine samples are the methods of prime interest.
organic halogens; halogenated organic compounds; determination methods; methodology10.17895/ices.pub.5036N/A
TextC. Grøn2707-6997978-87-7482-265-34/9/2019 12:03 PM
TIMES11.pdf
  
1991TimesBiological effects of contaminants: Oyster (Crassostrea gigas) embryo bioassay
11
4/20/2021 2:22 PMRuth Anderson
This paper describes the modified oyster embryo bioassay method which has been used by the Ministry of Agriculture, Fisheries and Food (MAFF), UK, to obtain a measure of the deterioration in biological water quality in UK coastal areas receiving anthropogenic discharges.
biological water quality; contaminants; oyster embryo; bioassays; methodology10.17895/ices.pub.5037N/A
TextJ. E. Thain2707-6997978-87-7482-266-04/9/2019 12:04 PM
TIMES12.pdf
  
1991TimesHydrocarbons: Review of methods for analysis in sea water, biota, and sediments
12
4/20/2021 2:29 PMRuth Anderson
This paper presents a review of methods for analysing hydrocarbons in seawater, biota and sediments. It is intended as an introduction to the subject and a collection of refrences from which a judicious choice has to be made based upon the specific objectives of an investigation.
hydrocarbon analysis; methodology; marine biota; marine sediment; seawater; aromatic hydrocarbons10.17895/ices.pub.5038N/A
TextM. Ehrhardt; J. Klungsøyr; R. J. Law2707-6997978-87-7482-267-74/9/2019 12:05 PM
TIMES13.pdf
  
1991TimesBiological effects of contaminants: Microplate method for measurment of ethoxyresorufin-O-deethylase (EROD) in fish
13
4/20/2021 2:37 PMRuth Anderson
Mixed function oxidase (MFO) can be used as a monitoring tool for measuring the effects of pollutants. The MFO system requires molecular O2 and NADPH and involves a co-binding protein: cytochromeP-450. The cytochrome P-450I A1 isozyme in fish can be induced by polycyclicaromatichydrocarbons (PAHs). Ethoxyresorufin-a-deethylase (EROD) activity is a specific assay for the xenobiotically inducible form of cytochrome P-450, thus making measurement of this activity in fish liver a good means of evaluating fish response to PAH contamination. This paper describes a method for routine measurements of EROD in flatfish. The method uses microplate technology and issuitable for use in the field on research vessels and in the laboratory.
contaminants; bioassays; ethoxyresorufin-O-deethylase; EROD10.17895/ices.pub.5039N/A
TextD. Burke; R. Mayer; A. Klotz; J. Stegeman; C. Walsh2707-6997978-87-7482-268-44/9/2019 12:06 PM
TIMES14.pdf
  
1991TimesTemporal trend monitoring: Introduction to the study of contaminant levels in marine biota
14
4/20/2021 7:39 PMRuth Anderson
The study of contaminants in marine species, sediments, and sea water has been of interest to the International Council for the Exploration of the Sea (ICES) since the early 1970s. The investigation of temporal trends (changes over time in one area) in contaminant levels in fish and shellfish, both as monitors of their environment and from a human health concern, and in sediments and sea water is a topic currently being addressed by the ICES Working Group on the Statistical Aspects of Trend Monitoring (WGSATM). This paper is intended to be the first of a series providing specific details for temporal trend tudies using different types of species or marine compartments. This first paper is a general one, describing the types of problems encountered in monitoring temporal trends in contaminant levels in finfish.
marine biota; temporal trend monitoring; contaminants10.17895/ices.pub.5040WGSATM
TextJ. F. Uthe; C. L. Chou; R. K. Misra; P. A. Yeats; D. H. Loring; C. J. Musial; W. Cofino2707-6997978-87-7482-269-14/9/2019 12:08 PM
TIMES15.pdf
  
1991TimesTemporal trend monitoring: Contaminant levels in tissues of Atlantic cod
15
4/20/2021 7:50 PMRuth Anderson
The study of contaminants in marine species, sediments, and sea water has been of interest to the International Council for the Exploration of the Sea (ICES) since the early 1970s. The investigation of temporal trends (changes over time in one area) in contaminant levels in fish and shellfish, both as monitors of their environment and from a human health concern, and in sediments and sea water is a topic currently being addressed by the ICES Working Group on the Statistical Aspects of Trend Monitoring (WGSATM). This is part of a series of leaflets and describes the Canadian techniques used to study temporal trends in contaminant levels, both tissue concentrations and burdens (tissue concentration times the total weight of that tissue in the  animal), inindividual Atlantic cod (Gadus morhua) muscle and liver tissues over the period1977-1985
atlantic cod; temporal trend monitoring; contaminants10.17895/ices.pub.5041WGSATM
TextJ. F. Uthe, R. K. Misra, C. L. Chou; D. P. Scott; C. J. Musial2707-6997978-87-7482-270-74/9/2019 12:09 PM
TIMES16.pdf
  
1991TimesBenthic communities: Use in monitoring point-source discharges
16
4/21/2021 8:57 AMRuth Anderson
Following a request from the ICES Advisory Committee on Marine Pollution (ACMP), the Benthos Ecology WorkingGroup (BEWG) undertook to report on the utility of field surveys of benthic fauna for monitoring the impact of point-source inputs (dumping or discharge of wastes). In response, BEWG have so far provided guidelines for benthic monitoring around discharge points. The present report revises and extends the coverage of these documents to include both a general appraisal of the utility of benthos studies in pollution monitoring, structured in line with the earlier ACMP request, and a detailed review of the use of meiofauna, in view of relatively recent developments in this field.
benthic communitities; monitoring; point source discharges10.17895/ices.pub.5042N/A
TextH. L. Rees; C. Heip; M. Vincx; M. M. Parker2707-6997978-87-7482-271-44/9/2019 12:52 PM
TIMES17.pdf
  
1996TimesNutrients: Practical notes on their determination in sea waterHAPISG
17
4/21/2021 11:35 AMRuth Anderson
These notes are aimed primarily at the freshwater chemist beginning to conduct analyses of nutrients in saline waters, but they will also be useful to the complete newcomer to the application of automated colorimetric techniques to natural waters in general. The emphasis is on automated techniques, but much of the material should be of interest to analysts who still use manual methods. The term 'nutrients' is a little difficult to define precisely, but from the point of view of the
marine chemist, phosphate, nitrate, nitrite, ammonia, and silicate are those most commonly encountered.
nutrients; seawater; sampling; marine chemistry10.17895/ices.pub.5043MCWG
TextD. Kirkwood2707-6997978-87-7482-272-14/9/2019 12:53 PM
TIMES18.pdf
  
1996TimesContaminants in marine organisms: Pooling strategies for monitoring mean concentrations
18
4/20/2021 8:09 PMRuth Anderson
Samples of marine organisms collected for contaminant monitoring are often pooled before being chemically analysed. However, there are several questions associated with pooling, including: what is an appropriate pooling strategy? how should data from pools be statistically analysed? how should results derived from pooled data be interpreted? This document is an introduction to the statistical aspects of pooling.
contaminants; marine organisms; pooling10.17895/ices.pub.5044WGSATM
TextM. D. Nicholson; R. J. Fryer2707-6997978-87-7482-274-54/9/2019 12:54 PM
TIMES19.pdf
  
1996TimesCommon diseases and parasites of fish in the North Atlantic: Training guide for identification
19
4/21/2021 8:28 AMRuth Anderson
During the past 20 years, there has been an increasing number of field surveys investigating the occurrence and distribution of fish diseases as a tool for monitoring the effects of environmental changes, including marine pollution. Fish diseases are considered to be an appropriate indicator in this context because the outbreak of a disease represents an end-point of biological significance
integrating all environmental factors affecting fish health.
This Training Guide gives advice to specialists and non-specialists on the following topics:
• fish species suitable for disease monitoring;
• sampling procedures;
• disease examination procedures;
• diseases useful for monitoring purposes and their diagnosis and classification;
• reporting of results;
• statistical methods applicable for data analysis
North Atlantic; parasites; diseases; identification10.17895/ices.pub.5045WGPDMO
TextD. Bucke; D. Vethaak; T. Lang; S. Mellergaard2707-6997978-87-7482-275-24/9/2019 12:56 PM
TIMES20.pdf
  
1998TimesTemporal trend monitoring: Robust method for analysing contaminant trent monitoring data
20
4/21/2021 8:57 AMRuth Anderson
This document describes a new method of assessing contaminant trends in fish muscle, fish liver, and shellfish. Previous methods of assessing trend data have often been complicated by the need to respond to unusual features of the data, either in the way they were collected, processed, or in their distribution. These complications are discussed in detail to show the reasoning behind the method described here, which aims to provide a simpler, robust and more complete method of analysing and presenting trends. Essentially, the method summarizes trends using a smoother, a specific class of smooth curves fitted to median log-concentrations. The theory and methodology of fitting smoothers is new and our knowledge of the performance of the fitted smoothers, particularly with small sample sizes, is only approximate. Although a preliminary application of the new method to the 1993 assessment of the data from the Joint Monitoring Programme of the Oslo and Paris Commissions was promising, more assessment and development of the method will be necessary. A very simple smoother is used here to make the computations and theory easy to follow. A detailed worked example is provided. Statistical theory and formulae are included as annexes.
contaminants; trend monitoring10.17895/ices.pub.5046WGSAEM
TextM. D. Nicholson; R. J. Fryer; J. R. Larsen2707-6997978-87-7482-276-94/9/2019 12:58 PM
TIMES21.pdf
  
1998TimesChlorobiphenyls in marine sediments: Guidelines for determination
21
4/21/2021 9:02 AMRuth Anderson
The analysis of chlorinated biphenyls in sediments generally includes extraction with organic solvents, clean-up, removal of sulphur, collum fractionation and gas chromatographic separation, mostly with electron capture detection. All of the steps in the procedure are susceptible to insufficient recovery and/or contamination. Different methods applied to each of
these steps are discussed with their advantages and disadvantages. Where possible, quality control procedures are recommended in order to check the method's performance. Gas chromatographic conditions are discussed with regard to injection, separation, detection and system performance. In addition, the quality control aspects relating to calibrants, extraction, and clean-up are considered. These guidelines are intended to encourage and assist analytical chemists to critically (re)consider their methods and to improve their procedures and/or the associated quality control measures, where necessary.
Chlorobiphenyls; marine sediments; guidelines10.17895/ices.pub.5047N/A
TextF. Smedes; J. de Boer2707-6997978-87-7482-277-64/9/2019 12:59 PM
TIMES22.pdf
  
1998TimesBiological effects of contaminants: Cholinesterase inhibitation by organophosphate and carbamate compounds
22
4/21/2021 9:08 AMRuth Anderson
The recent development of biomarkers based on the study of biological responses of organisms exposed to chemical contaminants has provided the biochemical tools essential to the implementation of programmes for monitoring the biological effects of contaminants. At the initiative of ICES and UNESCO-IOC study groups, several international workshops have advocated the development of biochemical methods for contaminant monitoring programmes in the marine environment. Among the major contaminants in the marine environment, insecticides have the potential to cause ecotoxicological effects because of their strong persistence (e.g., organochlorines) or high toxicity (e.g., organophosphates and carbamates). Although the transfer of these contaminants to the marine environment is generally diffuse and chronic, there are occasions when accidental discharges can cause ecological and economic impacts. A biomarker to monitor specifically the effects of organophosphates and carbamates is needed to facilitate the assessment of the discharge of these substances into the marine environment. Acetylcholinesterase (AChElEC 3.1.1.7.) inhibition has been used as a biomarker of the effects of organophosphate and carbamate compounds. This paper describes a method for the determination of acetylcholinesterase in invertebrate and vertebrate tissues and the use of this technique in monitoring.
contaminants; Cholinesterase inhibitation; organophosphates; carbamates10.17895/ices.pub.5048N/A
TextG. Bocquené; F. Galgani2707-6997978-87-7482-278-34/9/2019 1:00 PM
TIMES23.pdf
  
1998TimesBiological effects of contaminants: Determination of CYP1A-dependent mono-oxygenase activity in dab by fluorimetric measurement of EROD activity
23
9/30/2020 9:49 AMSøren Killerup Larsen
This paper describes a method for the determination of cytochrome P4501A (CYP1A) in fish liver by the measurement of 7-ethoxyresorufin O-deethylase (EROD) activity. The proposed method is by fluorescent assay of resorufin using internal standardization. The method is specifically for measurements made in dab (Limanda limanda L..), but is suitable for adaption to other species by small changes in assay conditions. The principle of the method, the sampling requirements, the assay procedures and the reporting of the results are described. Sources of error and quality control procedures are also specified.
contaminants; CYP1A-dependent mono-oxygenase; Dab; EROD activity; fluorimetric measurement; ethoxyresorufin-O-deethylase10.17895/ices.pub.5049N/A
TextR. Stagg; A. McIntosh2707-6997978-87-7482-279-04/9/2019 1:01 PM
TIMES24.pdf
  
1999TimesBiological effects of contaminants: Use of imposex in the dogwhelk (Nucella lapillus) as a bioindicator of tributyltin pollution
24
4/21/2021 9:12 AMRuth Anderson
This document describes a method for detecting contamination of the marine environment by tributyltin (TBT) using a sensitive neogastropod, the dogwhelk Nucella lapillus (L.), as a bioindicator. Exposure of female N. lapillus to TBT induces masculinization; this induction of masculinization has been termed 'imposex'. The indices that have been employed to measure imposex in N. lapillus are described here, together with a brief account of the biology of this organism.
contaminants; imposex; dogwhelk; tributyltin10.17895/ices.pub.5050N/A
TextP. E. Gibbs2707-6997978-87-7482-280-64/9/2019 1:02 PM
TIMES25.pdf
  
1999TimesBiological effects of contaminants: Measurement of DNA adducts in fish by 32P-postlabelling
25
4/21/2021 9:20 AMRuth Anderson
This document describes in detail the 32P-postlabelling method and its application to fish. Several recent studies have shown that the 32P-postlabelling method can be used to detect and measure the levels of DNA modified by large, hydrophobic aromatic compounds in teleosts. Moreover, the levels of hepatic DNA adducts in wild fish positively correlate with the concentrations of polycyclic aromatic compounds (PACs) present in marine sediments in several cases, and a strong positive correlation has been observed between sediment concentrations of PACs and the prevalence of neoplastic lesions in liver of marine flatfish. Laboratory studies with model PACs and sediment extracts also have shown that the PAC-DNA adducts formed are persistent and have chromatographic characteristics similar to DNA adducts detected in wild fish. These findings suggest that the levels of hepatic DNA adducts found in fish tissues can function as molecular dosimeters of exposure to potentially genotoxic environmental contaminants, such as high molecular weight PACs. The 32P-postlabelling assay has been used as a marker of exposure to potentially genotoxic contaminants in environmental
monitoring studies, such as NOAA's National Status and Trends (NS&T) Program and in the Bioeffects Surveys of NOAA's Coastal Ocean Program.
contaminants; DNA adducts; 32P-postlabelling10.17895/ices.pub.5051N/A
TextW. L. Reichert; B. L. French; J. E. Stein2707-6997978-87-7482-281-34/9/2019 1:03 PM
TIMES26.pdf
  
1999TimesBiological effects of contaminants: Quantification of metallothionein (MT) in fish liver tissue
26
4/21/2021 9:20 AMRuth Anderson
This document describes methods to analyse the protein metallothionein in fish tissues. Metallothionein is induced by and binds essential (Cu, Zn) and non-essential (Cd, Hg) metals
and is used in monitoring programmes as a marker for environmental metal exposure. The main focus is on the use and development of immunochemical procedures (ELISA). In addition, two alternative methods, electrochemical and spectrophotometric, are described.
contaminants; metallothionein; fish liver tissue10.17895/ices.pub.5053N/A
TextK. Hylland2707-6997978-87-7482-282-04/9/2019 2:08 PM
TIMES27.pdf
  
1999TimesSoft bottom macrofauna: Collection, treatment, and quality assurance of samples
27
4/21/2021 9:24 AMRuth Anderson
The aim of these recommendations is to standardize the methods used by different scientists for benthos surveys in order to increase the comparability of results for different areas. The results of ICES/HELCOM Quality Assurance workshops, intercalibrations, and ring tests have been incorporated in this set of recommendations in order to increase the quality, reliability and, thus, comparability of benthos data. This set of recommendations covers all steps from the design of the sampling programme to considerations of which gear to use, and all ship-board methods such as sampling with grabs, corers, dredges, and trawls. There is no single standard sampling gear for benthos investigations. The choice of a suitable sampler is a compromise between specific sampling characteristics in different sediment regimes in the area to be sampled, good handling characteristics at sea in bad weather conditions, suitability for various ships, financial limitations, tradition, and scientific questions. Criteria for the rejection of samples are identified. Treatment of samples is described in detail including sieving, transfer of the sample to the sample vessel, fixation, staining, and labelling, followed by a description of laboratory procedures such as sorting, taxonomic identification, and biomass determinations. A list of items for in-house quality assurance is included together with diagrams of suitable sieving devices and details for a warp-rigged Van Veen grab.
soft bottom macrofauna; samples; quality assurance10.17895/ices.pub.5054BEWG
TextH. Rumohr2707-6997978-87-7482-283-74/9/2019 2:10 PM
TIMES28.pdf
  
2001TimesBiological effects of contaminants: Corophium sp. sediment bioassay and toxicity test
28
6/12/2019 11:25 AMFfion Bell
contaminants; sediment bioassay; toxicity10.17895/ices.pub.5079N/A
TextB. D. Roddie; J. E. Thain4/11/2019 2:09 PM
TIMES29.pdf
  
2001TimesBiological effects of contaminants: Sediment bioassay using the polychaete Arenicola marina
29
4/21/2021 9:27 AMRuth Anderson
The method described here is a whole-sediment reworker bioassay using the polychaete Arenicola marina, a direct deposit feeder that is widely distributed in European coastal waters and on the east coast of North America. This method has been tested nationally in the UK as well as in ring tests under the Paris Commission. It is suitable for carrying out bioassays on field-collected sediments and also for toxicity testing. Bioassay endpoints include both mortality and a non-lethal indication of effect (inhibition of casting).
contaminants; sediment bioassay; toxicity10.17895/ices.pub.5055N/A
TextJ. Thain; S. Bifield2707-6997978-87-7482-284-44/9/2019 2:12 PM
TIMES30.pdf
  
2001TimesChlorophyll a: Determination by spectroscopic methods
30
6/12/2019 11:25 AMFfion Bell
chlorophyll a; determination methods; spectroscopy10.17895/ices.pub.5080N/A
TextA. Aminot; F. Rey4/11/2019 2:11 PM
TIMES31.pdf
  
2002TimesBiological effects of contaminants: Radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA) techniques for the measurement of marine fish vitellogenins
31
9/30/2020 12:23 PMSøren Killerup Larsen
This document describes immunochemical methods to quantify the egg-yolk precursor protein
vitellogenin in fish plasma. Vitellogenin is normally produced by the liver of mature female fish
in response to 17β-oestradiol (E2) in the blood. If male or reproductively immature fish are
exposed to oestrogenic substances, either in the water or the diet, their livers will also be
stimulated to produce vitellogenin. Concentrations of vitellogenin in the plasma of induced and
uninduced fish can differ by a factor of between 106 and 107. This makes vitellogenin induction
in male and immature fish a very good biomarker for environmental oestrogens. All necessary
steps in the development of both RIA (radioimmunoassay) and ELISA (enzyme-linked
immunosorbent assay) are described, as are special precautions that need to be considered
during the analysis of this protein.
contaminants; radioimmunoassay; enzyme-linked immunosorbent assay; RIA; ELISA; marine fish; vitellogenins10.17895/ices.pub.5081N/A
TextA. P. Scott; K. Hylland2707-6997978-87-7482-251-64/11/2019 2:12 PM
TIMES32.pdf
  
2004TimesBiological monitoring: General guidelines for quality assurance
32
4/21/2021 9:30 AMRuth Anderson
These   guidelines   have   been   prepared   by   the   ICES/OSPAR   Steering   Group   on   Quality   Assurance of Biological Measurements in the Northeast Atlantic (SGQAE), as part of its role to encourage the production of biological data of consistent quality by member countries. The  biological  measures  covered  are:  chlorophyll  a,  phytoplankton,  macrozoobenthos,  and  macrophytobenthos, reflecting the initial remit of the Steering Group to address eutrophication-related studies according to the specifications of the OSPAR Joint Assessment and Monitoring Programme  (JAMP).  Tables  of  critical  quality  assurance  (QA)  factors  and  priority  QA  actions  for  these  measures  are  presented.  However,  the  guidelines  for  developing  effective  QA/AQC  (analytical  quality  control)  procedures  governing  field  and  laboratory  work  will  be  found  to  have  a  more  general  relevance  to  laboratories  engaged  in  biological  studies  in  the  marine  environment. QA  guidelines  are  presented  across  the  full  range  of  monitoring  activities,  i.e.,  from  the  objective-setting  and  sampling  design  stages  of  field  surveys,  to  the  generation,  analysis,  and  archiving of data. Attention to all these activities is necessary in order to ensure the production of good quality information that continues to meet the purpose of scientific assessments. Where possible, illustrative examples of good practice in relation to QA of biological measures are  included.
biological montoring; quality assurance10.17895/ices.pub.5056N/A
Text2707-6997978-87-7482-252-34/9/2019 2:13 PM
TIMES33.pdf
  
2004TimesRecruitment studies: Manual on precision and accuracy of tools
33
4/21/2021 10:16 AMRuth Anderson
This manual is one of the results of the project Precision and Accuracy of Tools in Recruitment Studies (PARS), financed by the EU (FAIR-CT96–1371). The project PARS is concerned with improving the methodologies used in investigations and the monitoring of the early life stages of fish larvae, especially herring and sardine. These are important in both stock assessments and strategic research intended to improve predictive capability. The project deals with precision
and accuracy issues in two categories of measurements, which together encompass most of the data that are routinely required from samples of early life stages of fish: • the growth and condition of individuals; • the origin of individuals. This  manual  is  an  important  step  for  standardizing  techniques  used  in  recruitment  studies  and  will  be  beneficial  to  anyone  working  in  the  field  of  fish  recruitment  in  order  to  increase  the  knowledge of fish stocks and their management.
recruitment studies; precision; accuracy; tools10.17895/ices.pub.5057N/A
TextM. Belchier; C. Clemmesen; D. Cortes; T. Doan; A. Folkvord; A. Garcia; A. Geffen; H. Høie; A. Johannessen; E. Moksness; H. de Pontual; T. Ramirez; D. Schnack; B. Sveinsbo2707-6997978-87-7482-253-04/9/2019 2:14 PM
TIMES34.pdf
  
2004TimesBiological effects of contaminants: Quantification of δ-aminolevulinic acid dehydratase (ALA-D) activity in fish bloodHAPISG
34
4/21/2021 11:34 AMRuth Anderson
This document describes a colorimetric method to quantify the enzyme δ-aminolevulinic acid
dehydratase (ALA-D) in fish blood. ALA-D is an enzyme in the heme synthesis pathway. The activity of the enzyme is inhibited by lead (Pb) and it has, therefore, been used as a biomarker for lead exposure and effects in mammals, birds, and fish. This paper describes optimal conditions for the kinetic determination of ALA-D activity in fish blood, standardized to protein content.
contaminants; δ-aminolevulinic acid dehydratase; fish blood10.17895/ices.pub.5058MCWG
TextK. Hylland2707-6997978-87-7482-254-74/10/2019 12:52 PM
TIMES35.pdf
  
2004TimesChemical measurements in the Baltic Sea: Guidelines on quality assurance
35
4/21/2021 11:21 AMRuth Anderson
This document provides an introduction to quality issues, in general, and quality assurance in Baltic marine monitoring laboratories, in particular. The guidelines are intended to assist laboratories in starting up and operating their quality assurance systems. For laboratories with existing quality systems, the guidelines may give inspiration for issues that can be improved. The guidelines contain information for all levels of staff in the marine laboratory: organizational technical quality assurance principles that are relevant to administrative managers; implementation and operation of a quality system for quality managers; and for technical managers, the guidelines provide a description of the principles concerning how to introduce and maintain the technical aspects of quality assurance. These guidelines have been prepared by the ICES/HELCOM Steering Group on Quality Assurance of Chemical Measurements in the Baltic Sea (SGQAC)1 for use in association with the HELCOM Cooperative Monitoring in the Baltic Marine Environment (COMBINE) Programme, and the former Baltic Monitoring Programme.
Baltic Sea; quality assurance; marine chemistry10.17895/ices.pub.5059SGQAC
Text2707-6997978-87-7482-285-14/10/2019 12:54 PM
TIMES36.pdf
  
2004TimesBiological effects of contaminants: Measurement of lysosomal membrane stability
36
4/21/2021 11:26 AMRuth Anderson
Lysosomes are ubiquitous cellular organelles that provide a waste disposal and macromolecular recycling system (autophagy) and also a membrane-bound compartment for intracellular digestion of food ingested by the cells. They accumulate many toxic metals and organic chemical contaminants, providing an evolutionarily primitive detoxication capacity, which if overloaded results in lysosomal damage leading to cell injury, tissue dysfunction, and reduction in animal “health status”. Major reactions of lysosomes to pollutants include loss of membrane integrity, enlargement associated with autophagy, and accumulation of lipid and lipofuscin (agepigment). These types of responses have been widely used to test for the effects of toxic contaminants in both experimental investigations and environmental impact assessments. Several methods are available to measure lysosomal functional status: these include measurement of lysosomal membrane stability in both frozen tissue sections and live cells. Protocols for the implementation of these methods are described here in practical detail for mussel/molluscan digestive gland or hepatopancreas and flatfish liver. Cytochemically determined latency of selected lysosomal marker enzymes is used as the measure of stability in frozen sections, and retention time of the chromogenic dye neutral red, as the measure of lysosomal integrity in live cells. Guidelines are included for sample handling, data analysis, and interpretation of results.
contaminants; lysosomal membrane stability10.17895/ices.pub.5060N/A
TextM. N. Moore; D. Lowe; A. Köhler2707-6997978-87-7482-286-84/10/2019 12:55 PM
TIMES37.pdf
  
2004TimesBiological effects of contaminants: Use of intersex in the periwinkle (Littorina littorea)as a biomarker of tributyltin pollution
37
4/21/2021 11:34 AMRuth Anderson
The method described here is for detecting contamination of the marine environment by the biocide tributyltin (TBT) using the prosobranch snail Littorina littorea (L., 1758) as a bioindicator. This species is widely distributed in European coastal waters and on the east coast of North America. The method has been tested nationally in Germany as well as in international laboratory performance studies under the Joint Assessment and Monitoring Programme (JAMP) of the OSPAR Commission, as organized by QUASIMEME. Exposure of female L. littorea to
TBT induces a masculinization, which has been termed “intersex”. The indices that have been employed to measure intersex in L. littorea are described, together with a brief account of the biology of this organism.
contaminants; intersex; periwinkle; tributyltin10.17895/ices.pub.5061N/A
TextJ. Oehlmann2707-6997978-87-7482-288-24/10/2019 12:57 PM
TIMES38.pdf
  
2004TimesBiological effects of contaminants: Use of liver pathology of the European flatfish dab (Limanda limanda L.) and flounder (Platichthys flesus L.) for monitoring
38
4/21/2021 11:34 AMRuth Anderson
This publication provides quality assurance guidelines for the use of liver pathology of flatfish in biological effects of contaminants monitoring programmes. Information on the sampling procedures, including macroscopic examination, and minimum numbers and size categories of fish required to provide statistical rigour, is included. Details for laboratory processing and staining methods for histological assessment are also given, including protocols for resin embedding. Criteria for the histopathological diagnosis of liver sections are provided under categories on “early non-neoplastic toxicopathic lesions”, “foci of cellular alteration”, “benign neoplasms”, and “malignant neoplasms”, and descriptions of the normal appearance of liver tissue from dab (Limanda limanda) and flounder (Platichthys flesus) are included. More specific criteria are given for the different lesion types occurring in each of these categories. Each lesion type is represented by one or more colour micrographs taken from haematoxylin and eosinstained sections to depict the key features. A diagnostic key for nodular hepatocellular lesions is included and sections on quality assurance, data treatment, and data submission are also
provided.
contaminants; liver pathology; Dab; flounder; monitoring10.17895/ices.pub.5062N/A
TextS. W. Feist; T. Lang; G. D. Stentiford; A. Köhler2707-6997978-87-7482-289-94/10/2019 12:58 PM
TIMES39.pdf
  
2005TimesReview of analytical methods for determining metabolites of polycyclic aromatic compounds (PACs) in fish bile
39
9/30/2020 11:13 AMSøren Killerup Larsen
In fish, metabolites of polycyclic aromatic compounds (PACs) in gall bladder bile can be used as biomarkers for recent environmental exposure to PACs. These metabolites in the bile result from hepatic biotransformation processes whereby the lipophilic parent PACs are transformed in two steps (hydroxylation and subsequent conjugation) to more soluble forms and then passed to the gall bladder for elimination from the organism. As a biomarker of exposure, the determination of PAC metabolites in bile has several advantages over other assessment techniques. Several bile PACs are strong fluorophores and can thus be measured semi-quantitatively and very easily by means of straightforward fluorescence detection techniques. For example, fixed fluorescence detection and synchronous fluorescence scanning can be used for this purpose. These techniques are excellent for rapid screening of overall PAC exposure levels, but less suitable for the determination of individual compounds. The next level of resolution is reached with a high-performance liquid chromatographic (HPLC) separation of the conjugated bile metabolites prior to the fluorescence detection, so that individual metabolites and their patterns are discernable. Furthermore, PAC metabolites in bile can be enzymatically hydrolyzed to allow detection of free hydroxy PACs. After a centrifugation step the sample can be measured directly by HPLC/fluorescence (F). For analysis by gas chromatography/mass spectrometry (GC/MS) an extraction procedure is normally used to separate the hydroxy PACs from the bile matrix; derivatization can be used in order to increase separation and sensitivity. The latter set of methodological approaches can be used to determine individual metabolites, and a large number of hydroxy PACs are available as standards for accurate quantitation. In general, GC/MS methods are optimal for smaller compounds with 2–3 rings due to their better selectivity, while HPLC/F often provides better detection limits for larger metabolites with 4–5 rings. In the present review, the state-of-the-art for the various alternative methods is presented. Aspects of analytical quality control, interlaboratory comparability of data and the use of certified reference materials are also discussed. The advantages and limitations of each approach are discussed with respect to the use of PAC metabolites in bile as biomarkers of environmental PAC exposure in fish.
polycyclic aromatic compounds; fish bile; metabolite determination10.17895/ices.pub.5063N/A
TextF. Ariese; J. Beyer; G. Jonsson; C. P. Visa; M. M. Krahn2707-6997978-87-7482-291-24/10/2019 1:46 PM
TIMES40.pdf
  
2006TimesBiological effects of contaminants: Measurement of scope for growth in mussels
40
9/30/2020 11:14 AMSøren Killerup Larsen
Scope for growth (SFG) is a method of assessing the whole-animal physiological response to
sublethal stress induced by pollutants. It has been applied widely in small- and large-scale
pollution monitoring programmes in various regions of the world, ranging from temperate to
tropical. SFG was primarily developed for use with suspension-feeding mussels (Mytilus
edulis or similar indigenous species) and in combination with the analysis of chemical
contaminants in mussel tissues. SFG is based on the measurement of physiological responses,
such as feeding and respiration rate, and is derived from the difference between energy
acquisition (rate of feeding and digestion) and energy expenditure (metabolic rate). The
method has been successfully tested nationally in a range of UK monitoring programmes and
internationally as part of IOC (Intergovernmental Oceanographic Commission) Biological
Effects Workshops to evaluate and compare pollution effects measurements at different levels
of biological organization
contaminants; mussel10.17895/ices.pub.5064N/A
TextJ. Widdows; F. Staff2707-6997978-87-7482-292-94/10/2019 1:48 PM
TIMES41.pdf
  
2008TimesBiological effects of contaminants: The use of embryo aberrations in amphipod crustaceans for measuring effects of environmental stressors
41
9/30/2020 11:19 AMSøren Killerup Larsen
This report describes the methodology for assessing the proportions and different
types of embryo aberrations in both sediment‐dwelling and nektonic amphipods.
Determination of malformed embryos is a sensitive method of detecting the effects of
contaminants, such as trace metals and hydrophobic organic contaminants. Furthermore,
it is also possible to derive information about non‐contaminant environmental
effects, e.g. oxygen deficiency and temperature stress, by discriminating between different
types of embryo aberrations. Thus, the main advantage of the method is to
separate general effects of contaminants from other environmental stressors. It is a
general bio‐indicator that is sensitive to all kinds of xenobiotics and is applicable for
measuring effects of long‐term chronic impact of individual chemicals or mixtures of
contaminants, as well as acute local effects from point source discharges in situ.
contaminants; embryo aberrations; amphipod crustaceans; environmental stressors10.17895/ices.pub.5065N/A
TextB. Sundelin; A. K. Eriksson Wiklund; A. T. Ford2707-6997978-87-7482-293-64/10/2019 1:49 PM
TIMES42.pdf
  
2009TimesGuidelines for the study of the epibenthos of subtidal environments
42
9/30/2020 11:28 AMSøren Killerup Larsen
These Guidelines for the Study of the Epibenthos of Subtidal Environments document
a range of sampling gears and procedures for epibenthos studies that meet a
variety of needs. The importance of adopting consistent sampling and analytical
practices is highlighted. Emphasis is placed on ship‐based techniques for surveys of
coastal and offshore shelf environments, but diver‐assisted surveys are also considered.
The account extends earlier work by the ICES Benthos Ecology Working Group on
methods for studying the benthic communities of hard substrata (Connor, 1995; see
Annex 1). It also complements the ICES Techniques in Marine Environmental Sciences
(TIMES) guidelines for the study of the soft‐bottom macrofauna (Rumohr, in prep.),
the phytobenthos (Kautsky, in prep.), and other publications dealing with benthic
sampling methods, notably Eleftheriou and McIntyre (2005).
Coverage of sampling gears is not exhaustive, and others may be added in future editions.
The target audience includes marine scientists new to epibenthic studies as well
as established practitioners who require further detail on sampling practices in order
to meet various objectives of contemporary interest.
epibenthos; subtidal environments10.17895/ices.pub.5066BEWG
TextM. J. N. Bergman; S. N. R. Birchenough;  Á. Borja; S. E. Boyd; C. J. Brown; L. Buhl-Mortensen; R. Callaway; D. W. Connor; K. M. Cooper; J. Davies; I. de Boois; K. D. Gilkinson; D. C. Gordon Jr; H. Hillewaert; H. Kautsky; M. de Kluyver; I. Kröncke; D. S. L2707-6997978-87-7482-294-34/10/2019 1:50 PM
TIMES43.pdf
  
2009TimesSoft-bottom macrofauna: Collection, treatment, and quality assurance of samples
43
9/30/2020 11:27 AMSøren Killerup Larsen
The results of ICES/HELCOM Quality Assurance workshops, intercalibrations, and
ring tests have been incorporated into this set of recommendations to increase the
quality, reliability and, therefore, comparability of benthos data before their final
evaluation and storage in public databanks. These recommendations are timely,
given the increasing number of researchers and institutions that are engaged in
sorting and analysing benthos samples. This document covers all steps, from the
design of the sampling programme, to considerations of which gear to use, as well as
all shipboard methods, such as sampling with grabs, corers, dredges, and trawls.
There is no single, standard sampling gear for benthos investigations. The choice of
an appropriate sampler depends on the average living depth of the infauna of
interest; this depth can range from the uppermost millimetre down to almost 1 m.
When analysing the results, possible discrepancies between the penetration depth of
the sampler and the actual living depth of the organisms must be considered. The
choice of a suitable sampler is a compromise between specific sampling
characteristics in different sediment regimes in the area to be sampled, good handling
characteristics at sea in bad weather conditions, suitability for various ships, financial
limitations, tradition, and scientific questions posed. Criteria for the rejection of
samples are identified. Treatment of samples is described in detail, including sieving,
transferring of the sample to the sample vessel, fixation, staining, and labelling.
Laboratory procedures for sorting, taxonomic identification, and biomass
determination are described. A list of items for in‐house quality assurance is
included, as well as details for a warp‐rigged van Veen grab
soft bottom macrofauna; samples; quality assurance10.17895/ices.pub.5067BEWG
TextH. Rumohr2707-6997978-87-7482-295-04/10/2019 1:51 PM
TIMES44.pdf
  
2009TimesDetermination of Hexabromocyclododecane (HBCD) in sediment and biota
44
9/30/2020 11:27 AMSøren Killerup Larsen
This document provides advice on the analysis of hexabromocyclododecane (HBCD)
in sediment and biota. The analysis of HBCD in sediment and biota generally
involves extraction with organic solvents, clean‐up, and either gas chromatographic
separation with mass‐spectrometric (MS) detection or liquid chromatography with
MS detection. All stages of the procedure are susceptible to insufficient recovery
and/or contamination. Where possible, quality‐control procedures are recommended
to check the method’s performance. These guidelines are intended to encourage and
assist analytical chemists to reconsider their methods and to improve their
procedures and/or the associated quality‐control measures where necessary.
Keywords: hexabromocyclododecane, sediment, biota, sample pretreatment, storage,
extraction, clean‐up, calibration, liquid chromatography, mass spectrometry.
Hexabromocyclododecane; HBCD; sediment; biota10.17895/ices.pub.5069MCWG
TextL. Webster; P. Bersuder; J. Tronczynski; K. Vorkamp; P. Lepom2707-6997978-87-7482-296-74/11/2019 11:40 AM
TIMES45.pdf
  
2009TimesDetermination of parent and alkylated polycyclic aromatic hydrocarbons (PAHs) in biota and sediment
45
9/30/2020 12:01 PMSøren Killerup Larsen
This document provides advice on the analysis of parent and alkylated polycyclic
aromatic hydrocarbons (PAHs) in total sediment, sieved fractions, suspended
particulate matter, and biota (shellfish). The determination of parent and alkylated
PAHs in sediment and biota includes extraction with organic solvents, clean‐up, and
analysis by gas chromatography (GC) with mass spectrometry (GC‐MS). Advice is
given on the treatment and storage of samples. Extraction and clean‐up methods
commonly used are described. GC‐MS is the only recommended method for the
analysis of both parent and alkylated PAHs and advice is provided on standards and
calibration. All steps in the procedure are susceptible to insufficient recovery and/or
contamination. Quality‐control procedures are recommended to check the
performance of the method. These guidelines are intended to encourage and assist
analytical chemists to reconsider their methods critically and to improve their
procedures and/or the associated quality‐control measures, where necessary.
parent and alkylated polycyclic aromatic hydrocarbons; PBDEs; sediment; biota10.17895/ices.pub.5070MCWG
TextL. Webster; J. Tronczynski; P. Korytar; K. Booij; R. Law2707-6997978-87-7482-297-44/11/2019 11:42 AM
TIMES46.pdf
  
2009TimesDetermination of polybrominated diphenyl ethers (PBDEs) in sediment and biota
46
9/30/2020 12:02 PMSøren Killerup Larsen
This document provides advice on the analysis of polybrominated diphenyl ethers
(PBDEs) in biota and sediment. The determination of PBDEs in sediment and biota
generally involves extraction with organic solvents, clean‐up, and gas
chromatographic separation with mass‐spectrometric detection. All stages of the
procedure are susceptible to insufficient recovery and/or contamination. Therefore,
quality‐control procedures are important to check the method’s performance. These
guidelines are intended to encourage and assist analytical chemists to reconsider
their methods and to improve their procedures and/or the associated quality‐control
measures where necessary.
polybrominated diphenyl ethers; PBDEs; biota; sediment10.17895/ices.pub.5071MCWG
TextL. Webster; J. Tronczynski; P. Bersuder; K. Vorkamp; P. Lepom2707-6997978-87-7482-298-14/11/2019 11:43 AM
TIMES47.pdf
  
2010TimesMonitoring organotins in marine biota
47
9/30/2020 12:02 PMSøren Killerup Larsen
These guidelines provide best practices for the measurement of organotin compounds
in biota for monitoring programmes. Target compounds include tributyltin
(TBT), dibutyltin (DBT), and monobutyltin (MBT) as well as triphenyltin (TPhT), diphenyltin
(DPhT), and monophenyltin (MPhT). The bivalve Mytilus edulis can be a
suitable target species for the monitoring of organotins. Sampling strategy as well as
transportation and storage are important for the final quality of the data. Handling
and pretreatment of the samples is also discussed. Several analytical methods can be
used for the determination of organotin compounds. Critical steps, such as extraction
and derivatization of the determinants, are discussed, followed by descriptions of the
analytical detection techniques. Emphasis is placed on analytical quality control and
quality assurance.
organotins; marine biota10.17895/ices.pub.5072MCWG
TextE. Monteyne; J. Strand; K. Vorkamp; P. Bersuder; T. Bolam; M. Giltrap; E. McGovern2707-6997978-87-7482-299-84/11/2019 11:43 AM
TIMES48.pdf
  
2010TimesDetermination of perfluoroalkyl compounds in water, sediment, and biota
48
9/30/2020 12:04 PMSøren Killerup Larsen
This document provides advice on the analysis of polyfluoroalkyl compounds (PFCs)
in samples of water, sediment, and biota. The analysis of PFCs in these matrices
generally includes extraction with organic solvents, clean‐up, and liquid
chromatography (LC) with mass spectrometric (MS) detection. This document
provides an overview of environmentally relevant PFCs and information on the
currently applied techniques for the analysis of these PFCs, including sampling,
pretreatment, extraction, clean‐up, instrumental analysis, quantification and quality
assurance, and quality control.
perfluoroalkyl compounds; water; sediment; biota10.17895/ices.pub.5073WGMS
TextL. Ahrens; K. Vorkamp; P. Lepom; P. Bersuder; N. Theobald; R. Ebinghaus; R. Bossi; J. L. Barber; E. McGovern2707-6997978-87-7482-300-14/11/2019 11:44 AM
TIMES49.pdf
  
2012TimesDetermination of alkylphenol metabolites in fish bile using solid phase analytical derivatizantion (SPAD) and gas chromatography-mass spectrometry in electron ionization mode (GC-EI-MS)
49
9/30/2020 12:04 PMSøren Killerup Larsen
This document provides advice on the analysis of alkylphenol (AP) metabolites in
fish bile. APs are released to aquatic environments from many different sources
related to human activities, such as offshore oil production. The method for
determination of APs includes enzymatic deconjugation of fish bile followed by
solid‐phase analytical derivatization (SPAD) with bis(trimethylsilyl)‐
trifluoroacetamide (BSTFA). The derivatized APs are separated, then quantified
using gas chromatography–mass spectrometry in the electron ionization mode (GC–
EI–MS). Quality control measures should be implemented to ensure good
performance of the method. This GC–EI–MS method allows for a selective and
sensitive analytical detection of APs in fish bile.
alkylphenol metabolites; fish bile; solid phase analytical derivatizantion; SPAD; gas chromatography-mass spectrometry in electron ionization mode; GC-EI-MS10.17895/ices.pub.5074N/A
TextG. Jonsson; A. Nævdal; J. Beyer2707-6997978-87-7482-301-84/11/2019 11:46 AM
TIMES50.pdf
  
2012TimesDetermination of polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans, and dioxin-like polychlorinated biphenyls in biota and sediment
50
9/30/2020 12:17 PMSøren Killerup Larsen
Polychlorinated dibenzo‐p‐dioxins, polychlorinated dibenzofurans (PCDD/Fs) and
polychlorinated biphenyls (PCBs) are environmental contaminants regulated by the
Stockholm Convention of Persistent Organic Pollutants. Being hydrophobic and
lipophilic, these compounds accumulate in the marine environment in sediments and
lipid‐rich tissue of marine organisms, making these matrices preferred media for
environmental monitoring. This document focuses on the analysis of PCDD/Fs and
dioxin‐like PCBs (i.e. non‐ortho and mono‐ortho PCBs), which have a similar planar
molecular structure to PCDD/Fs and, therefore, exhibit similar toxic effects. Because
concentrations in the environment are low and common analytical methods result in
co‐extractions of a large variety of potentially interfering compounds, analytical
procedures are complex. This document includes comments and advice on sampling
and sample pretreatment steps, suitable extraction and clean‐up procedures as well
as preconcentration methods. It highlights the importance of extract clean‐up and the
risk of contamination. Furthermore, suitable methods for instrumental analysis are
discussed for gas chromatographic separation, compound identification, and
quantification and detection methods. Although high‐resolution mass spectrometry
often is the method of choice, low‐resolution mass spectrometry can also provide
sufficiently sensitive analyses, in particular for screening purposes. In this context,
bioassays can also play a role, reflecting a cumulative toxicity rather than
concentrations of individual congeners. The paper also discusses general aspects of
good laboratory practice, quality assurance/quality control, and laboratory safety.
polychlorinated dibenzo-p-dioxins; polychlorinated dibenzofurans; biota; sediment10.17895/ices.pub.5075MCWG
TextK. Vorkamp; P. Roose; P. Bersudar; L. Webster; P. Lepom; C. Munschy; R. Bossi; J. Tronczynski; J. de Boer2707-6997978-87-7482-302-54/11/2019 11:47 AM
TIMES51.pdf
  
2012TimesBiological effects of contamination: Paracentrotu lividus sea urchin embryo test with marine sediment elutriates
51
9/30/2020 12:17 PMSøren Killerup Larsen
This ICES Techniques in Marine Environmental Sciences describes a sediment elutriate
bioassay using embryos of the sea urchin Paracentrotus lividus, a species widely
distributed in both Atlantic and European Mediterranean waters. The proposed
method is directly applicable to other echinoid species used in ecotoxicology
worldwide, such as the Strongylocentrotus and Arbacia genus. The bioassay endpoint
is a quantitative, observer‐independent, automatically readable response. Statistical
methods and assessment criteria to classify sediment samples according to their
biological quality status are also included, consistent with the demands of the
European Water Framework Directive.
contaminants; sea urchin; embryo; marine sediment10.17895/ices.pub.5076WGBEC
TextR. Beiras; I. Durán; J. Bellas; P. Sánches-Marín2707-6997978-87-7482-303-24/11/2019 11:48 AM
TIMES52.pdf
  
2012TimesGuidelines for passive sampling of hydrophobic contaminants in water using silicon rubber samplers
52
9/30/2020 12:19 PMSøren Killerup Larsen
This ICES Techniques in Marine Environmental Sciences provides advice on the use of
silicone rubber passive samplers for the determination of freely dissolved non‐polar
contaminants in seawater. The level of detail offered may be helpful to first‐time
users of passive samplers, who wish to implement passive sampling methods in their
monitoring programmes, and to more experienced users to review their current
methods. The aspects covered by these guidelines include pre‐extraction, spiking
with performance reference compounds, deployment, retrieval, extraction, clean‐up,
chemical analysis, and calculation of aqueous concentrations.
passive sampling; hydrophobic contaminants; water; silicon rubber samplers10.17895/ices.pub.5077N/A
TextF. Smedes; K. Booij2707-6997978-87-7482-304-94/11/2019 11:49 AM
TIMES53.pdf
  
2013TimesDetermination of polychlorianted biphenyls (PCBs) in sediment and biota
53
9/30/2020 12:21 PMSøren Killerup Larsen
Polychlorinated biphenyls (PCBs) are environmental contaminants regulated by the Stockholm Convention of Persistent Organic Pollutants (POPs), and are included on the OSPAR List of Chemicals for Priority Action due to their persistence, potential to bioaccumulate, and toxicity. Analysis of the ICES-7 PCBs (CB28, 52, 101, 118, 138, 153, and 180), in sediment and biota, is a mandatory requirement of the OSPAR Co-ordinated Environmental Monitoring Programme (CEMP). Three of the four non-ortho (CB77, 126 and 169) are classed as pre-CEMP determinands at the time of publication, and analysis in biota is recommended but on a voluntary basis.
This document provides advice on the analysis of PCBs in biota and sediment, including non-ortho PCBs. The determination of PCBs in sediment and biota generally involves extraction with organic solvents, clean-up, and gas chromatographic separation with electron capture detection or mass spectrometry. Due to the low concentrations of non-ortho substituted PCBs compared to those of other PCBs, their determination may require an additional separation step.
All stages of the procedure are susceptible to insufficient recovery and/or contamination. Therefore, quality control procedures are important in order to check method performance. These guidelines have been prepared by members of the ICES Marine Chemistry Working Group (MCWG) and the Working Group on Marine Sediment (WGMS) and are intended to encourage and assist analytical chemists to reconsider their methods and to improve their procedures and/or the associated quality control measures where necessary.
Keywords: polychlorinated biphenyls, sediment, biota, storage, extraction, clean-up, calibration, gas chromatography, electron capture detection, mass spectrometry.
polychlorianted biphenyls; PCBs; sediment bioassay; biota10.17895/ices.pub.5078MCWG
TextL. Webster; L. Roose; P. Bersuder; m. Kotterman; M. Haarich; K. Vorkamp2707-6997978-87-7482-305-64/11/2019 11:50 AM
TIMES54.pdf
  
2013TimesOyster embryo-larval bioassay (revised)
54
9/30/2020 12:22 PMSøren Killerup Larsen
The description of the oyster (Crassostrea gigas) embryo bioassay was initially published in the ICES TIMES series in 1991 (No.11). At the time, the bioassay was used in the United Kingdom for measuring water quality in relation to coastal waters which received anthropogenic discharges. Subsequently it was applied to measure general water quality and was taken up by the OSPAR Joint Assessment Monitoring Plan (JAMP) as a technique for measuring general biological effects in water, sediment elutriates, and pore water, and is used in several countries across the OSPAR Maritime Area.
The organism response used in the bioassay is the ability of early stage embryos to develop normally and reach the “D-shaped” larval stage (at which the paired hinged shells can be seen) within 24 hours. Although the exposure time is short, it encompasses a period of intense cellular activity during which the impairment of a number of critical physiological and biochemical processes may result in poor and abnormal growth and development.
The method described here is a complete revision of the original text, and includes a more comprehensive description of the methodology, how it can be applied for testing water quality for monitoring purposes, and for direct toxicity assessment or where a dilution series may need testing (e.g. extracts from passive samplers / sediment elutriates etc). Furthermore, there is additional information on the treatment and work up of results and analytical quality control.
oyster; embryo; larval; bioassay10.17895/ices.pub.5082N/A
TextD. Leverett; J. Thain2707-6997978-87-7482-307-04/11/2019 2:13 PM
TIMES55.pdf
  
2013TimesProtocol for measuring dioxin-like activity in environmental samples using in vitro reporter gene DR-Luc assays
55
9/30/2020 12:23 PMSøren Killerup Larsen
Guidelines are given for the quantification of the dioxin-like activities of contaminants in sediment, biota and water samples using the DR-Luc reporter gene bioassay. Dioxins and dioxin-like compounds demonstrate high affinity binding to the Aryl hydrocarbon Receptor (AhR). Ah-R is a ligand-activated transcription factor and mediates most, if not all, of the toxic responses of polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs), coplanar polychlorinated biphenyls (PCBs), and polybrominated biphenyls (PBBs). The DR-Luc bioassay, or DR-CALUX® (Dioxin Response Chemical Activated LUciferase gene eXpression), utilizes a recombinant rat hepatoma H4IIE cell line with a stably integrated AhR-responsive luciferase reporter gene. Exposure of this bioassay to extracts containing dioxin-like compounds induces the enzyme luciferase in a time, dose, and chemical specific manner. Cells are cultured in the laboratory and transferred to 96-well plates. Luciferase activity is determined by measuring the light emitted, which is directly proportional to the amount of dioxin-like compounds within the test extract. Hence the DR-Luc assay is a rapid, extremely sensitive and cost-effective tool for screening sediment, biota, and water extracts for dioxins and dioxin-like compounds. The DR-Luc assay is recommended in the OSPAR JAMP guidelines as a specific biological effect method for monitoring of PCBs, polychlorinated dibenzodioxins and furans, and also as a suitable biological effect method for general biological effect monitoring. In addition, DR-Luc analysis has proven to be a very powerful tool in emission source monitoring and remediation efforts as it allows for the identification and control of the toxic compounds concerned. Critical steps, such as the extraction of sediment or biota samples and subsequent clean-up of the extracts are discussed, followed by descriptions of the DR-Luc detection technique. Emphasis is placed on analytical quality control and quality assurance.
dioxin-like activity; measurement; samples; in vitro reporter gene DR-Luc assays10.17895/ices.pub.5083WGBEC
TextC. A. Schipper; P. E. G. Leonards; H. J. C. Klamer; K. V. Thomas; A. D. Vethaak2707-6997978-87-7482-131-14/11/2019 2:14 PM
TIMES56.pdf
  
2015TimesLysosomal membrane stability in mussels
56
9/30/2020 12:24 PMSøren Killerup Larsen
In 2012, the ICES Study Group on Integrated Monitoring of Chemicals and their Effects
provided a framework for integrated monitoring to the OSLO-Paris Commission.
UNEP/MAP and HELCOM expert groups have also developed guidelines on
integrated monitoring of chemicals and their effects for the Mediterranean and Baltic
Sea. This document provides the technical information for one of the biological effects
measurements, the lysosomal membrane stability (LMS), which is a part of the above
mentioned integrated monitoring approaches. Lysosomes are cytoplasmic, single
membrane organelles whose condition is sensitive to stress whether it be due to environmental
conditions or exposure to a wide array of contaminants. Two different
methodologies have been developed to assess LMS in mussels: an enzyme cytochemical
method using cryostatic sections of digestive gland tissue, and an in vivo cytochemical
method (using haemolymph cells). In this document, different aspects of
the operational procedures have been standardized and harmonized, with particular
reference to the in vivo cytochemical method. New graphical material has been added
to clarify criteria of interpretation and new external quality assurance programmes
for measurements of lysosomal membrane stability have been proposed. Background
(BAC) and environmental (EAC) assessment criteria to assess the LMS data are provided.
Additionally, a new scoring procedure to enhance the sensitivity of the LMS
measurements using the in vivo assay is provided.
lysosomal membrane stability; mussel10.17895/ices.pub.5084WGBEC
TextC. Martínez-Gómez; J. Bignell; D. Lowe2707-6997978-87-7482-166-34/11/2019 2:15 PM
TIMES57.pdf
  
2016TimesDetermination of CYP1A-dependent mono-oxygenase activity in dab by fluorimetric measurement of EROD activity in S9 or microsomal liver fractions
57
9/30/2020 12:24 PMSøren Killerup Larsen
This paper describes a method for the determination of cytochrome P4501A (CYP1A)
in fish liver fractions by the measurement of 7-ethoxyresorufin O-deethylase (EROD)
activity. The proposed method is by fluorescent assay of resorufin using internal standardization.
The method is specifically for measurements made in dab (Limanda limanda
L.), but is suitable for adaptation to other species. The principle of the method, the
sampling requirements, the assay procedures and the reporting of the results are described.
Sources of error and quality control procedures are also specified. The document
is a modification of a 1998 publication to allow measurement of EROD activity
from both S9 and microsomal fractions to be undertaken.
CYP1A-dependent mono-oxygenase activity; Dab; fluorimetric measurement of EROD activity; ethoxyresorufin-O-deethylase; S9; liver10.17895/ices.pub.5085WGBEC
TextR. Stagg; A. McIntosh; M. J. Gubbins2707-6997978-87-7482-168-74/11/2019 2:16 PM
TIMES58.pdf
  
2016TimesBiological effects of contaminants: Assessing DNA damage in marine species through single-cell alkeline gel electrophoresis (comet) assay
58
9/30/2020 12:26 PMSøren Killerup Larsen
Single cell gel electrophoresis (SCGE or comet) assay allows quantification of DNA
damage in individual cells and is an ideal tool for use within biological monitoring
programmes. Comet assay can be used on a range of cell types including somatic, reproductive
(gametes) or circulatory cells in many different species including both marine
bivalves and flatfish. The assay can be employed with simple equipment available
in most laboratories, is sensitive to environmentally relevant levels of DNA damage
(Frenzilli and Lyons, 2009), accurately demonstrates a linear dose response to exposure
(Collins et al., 1996), and can be adapted for use on most nucleated cell types. This document
concentrates on the simplest and most repeatable method of comet assay in circulatory
cells of species commonly used in marine biomonitoring programmes both
for chemical and biological effects. This manuscript describes standardized assay procedures
and recommends the minimum level of information required when reporting
comet assay results.
contaminants; DNA damage; single-cell alkeline gel electrophoresis (comet) assay10.17895/ices.pub.5086WGBEC
TextT. P. Bean; F. Akcha2707-6997978-87-7482-186-14/11/2019 2:17 PM
TIMES59.pdf
  
2019TimesBiological effects of contaminations: Stress on stress (SoS) response in mussels
59
9/30/2020 12:28 PMSøren Killerup Larsen
It is well known that the physiological status of marine organisms changes when they
are exposed to contaminants (Bayne et al., 1986; De Zwaan et al., 1995; Viarengo et al.,
1995). One consequence is that the organism is less able to tolerate the natural fluctuations
of environmental factors. Mussels can tolerate aerial exposure for many days but,
under sustained aerial exposure, they will eventually die. The ability of mussels to keep
valves closed and to resist aerial exposure relates to the amount of adenosine triphosphate
(ATP) available to fuel the adductor muscle (De Zwaan and Mathiew, 1992). In
mussels from contaminated sites, part of the metabolic energy is spent on detoxification
processes, thus depleting the ATP needed for other physiological functions. The
reduction of survival in air, or stress on stress (SoS) biomarker, is a simple and lowcost
whole organism response that can show pollutant induced alterations in the organism’s
physiology that renders the animal more sensitive to further environmental
changes.
Different studies have demonstrated the applicability of aerial survival as an early
warning indicator of contaminant-induced stress. The effects of xenobiotics, including
heavy metals, organometals, and organics, as well as contaminated field sediments, on
invertebrate survival in air have been demonstrated (De Zwaan et al., 1996). Bivalve
molluscs have been used in most studies, with marine mussels (Mytilus sp.) being the
most common organism (Brooks et al., 2018; Eertman et al., 1995; Smaal et al., 1991;
Veldhuizen-Tsoerkan et al., 1991; Thomas et al., 1999; Petrovic et al., 2004; Viarengo et
al., 1995; Pampanin et al., 2005; Labarta et al., 2005; Gorbi et al., 2008; Marcheselli et al.,
2011). Laboratory studies have been conducted to establish the relationships between
toxicant concentrations in tissue and SoS. For example, it was demonstrated that shortterm
exposure to sublethal concentrations (less than μM) of pollutants such as Cu2+,
DMBA (9, 10-dimethyl 1, 2 benzanthracene), and Aroclor 1254 significantly reduced
the capacity of mussels to survive in air (Viarengo et al., 1995). This effect was markedly
dose-dependent, and was strongly increased by pollutant mixtures, such as Cd and
PCB 126 (Viarengo et al., 1995; Eertman et al., 1996). Clams exposed to high concentrations
of 4-nonylphenol (3 mg NP l−1) were also found to have a significant decreased
ability to survive in air (Matozzo et al., 2003). A marked decrease in tolerance to aerial
exposure has also been reported in mussels exposed to high concentrations of the antifouling
biocide zinc pyrithione (ZnPT) (Marcheselli et al., 2011). Aerial exposure tolerance,
as a monitoring tool, has been reported as better able to reflect smaller differences
between mussels from sewage outfall sites and mussels from reference sites than other
physiological measurements, such as byssal thread production (Moles and Hale, 2003).
Survival in air measurements also appear to be a sensitive and statistically significant
parameter for monitoring the effect of long-term exposure to crude oil (Thomas et al.,
1999).
contaminants; stress on stress; SoS; mussel10.17895/ices.pub.4702N/A
TextJ. Thain; B. Fernández; C. Martínez-Gómez2707-6997978-87-7482-226-44/11/2019 2:19 PM
TIMES60.pdf
  
2017TimesSupporting variables for biological effects measurements in fish and blue mussels
60
9/30/2020 12:29 PMSøren Killerup Larsen
Biological effects measurements in fish and blue mussel are fundamental in marine
environmental monitoring. Nevertheless, currently used biomarkers may be confounded
by basic physiological phenomena, such as growth, reproduction, and feeding,
as well as thereby associated physiological variation. Here, we present a number
of supporting variables, which are essential to measure in order to obtain reliable biological
effects data, facilitate their interpretation, and make valid comparisons. For fish,
these variables include: body weight, body length, condition, gonad maturation status,
various somatic indices, age, and growth. For blue mussels, these variables include:
volume, flesh weight, shell weight, and condition. Also, grossly visible anomalies, lesions,
and parasites should be recorded for both fish and blue mussels. General confounding
factors and their effects are described, as well as recommendations for how
to handle them.
biological effects measurement; fish; blue mussels10.17895/ices.pub.2903WGPDMO
TextT. Hansson; J. Thain; C. Martínez-Gómez; K. Hylland; M. Gubbins; L. Balk2707-6997978-87-7482-200-44/11/2019 2:20 PM
TIMES61.pdf
  
2017TimesGuidelines for determining polymer-water and polymer-polymer partition coefficients of organic compounds
61
9/30/2020 12:29 PMSøren Killerup Larsen
Methods for the experimental determination of polymer-water partition coefficients
(Kpw) and polymer-polymer partition coefficients (Kp1p2) are reviewed with the aim to
improve the quality of passive sampling-based monitoring of organic compounds.
Mechanistic models are used for optimizing the experimental design of Kpw
measurements with respect to scaling (polymer mass, water volume, concentration
levels) and equilibration times. It is shown that the polymer-water phase ratio has a
profound effect on the rate of equilibrium attainment. Experimental artefacts are
discussed and quality control measures for quantifying uncertainties in the reported
Kpw values are suggested. Examples of experimental design modelling are provided.
Experimental methods for determining Kp1p2 are not fully developed yet and several
suggestions for the further development of Kp1p2 measurements are included. It is
expected that this guideline will be useful for investigators who seek to improve their
experimental procedures for determining polymer-water and polymer-polymer
partition coefficients, or to assess the quality of literature values of these partition
coefficients.
polymer-polymer; polymer-water; partition coefficients; organic compounds10.17895/ices.pub.3285MCWG
TextK. Booij; F. Smedes; I. J. Allan2707-6997978-87-7482-203-54/11/2019 2:21 PM
TIMES62.pdf
  
2019TimesGuidelines for the use of Diffusive Gradients in Thin Films for measuring metal fluxes in sedimentHAPISG
62
4/7/2021 8:04 AMRuth Anderson
Increasing public and regulatory concern over the chemical and ecological status of
water bodies has led to a higher demand for performing extensive water quality assessments.
However, regulatory frameworks often only focus on monitoring contaminants
in the water column, and scarce regulatory attention has so far been dedicated to
assessing sediment quality. Contaminants accumulated in the sediment may be released
into the overlying water, or reach higher trophic levels due to transfer across the
aquatic food chain. Thus, quality assessments of water bodies should include an evaluation
of the occurrence of contaminants in sediments and the associated ecological
risk.
During the last decades, efforts have been made to integrate sediment quality assessments
into regulatory frameworks. As a consequence, robust and reliable methodologies
for assessing sediment quality are now required. Among the existing methods for
assessing metal contamination in sediments, the Diffusive Gradients in Thin films
(DGT) technique has shown potential, due to its ability to measure metal fluxes from
the pore water and labile sediment phases. Laboratory and field studies indicate that
the DGT-labile metal flux provides robust predictions of metal bioaccumulation and
toxicity to benthic organisms (Roulier et al. 2008; Dabrin et al. 2012; Simpson et al. 2012;
Amato et al. 2014, 2015, 2016, 2018; He et al. 2018). DGT could thus be a useful tool for
improving sediment quality assessments
contaminants; sediment; DGT; metal fluxes; water quality10.17895/ices.pub.5463WGMS
TextAmato, E.D.; Bolam, T.; and Belzunce-Segarra, M.J.2707-6997978-87-7482-241-712/20/2019 10:41 AM
TIMES63.pdf
  
2020TimesProtocol for the verification of ballast water compliance monitoring devicesHAPISG
63
9/30/2020 12:31 PMSøren Killerup Larsen
Concerns regarding the impacts of non-native species due to their transport and release in ship ballast water have resulted in agreements and regulatory requirements being implemented around the world (e.g. International Maritime Organization [IMO] Ballast Water Management Convention [BWMC], 2004; US Coast Guard [USCG], 2012; California State Lands Commission [CSLC], 2018). Consequently, effective and reliable monitoring for ship compliance with ballast water discharge standards is now critical to achieve the regulatory goal of minimizing the risk of invasive species introductions. A variety of ballast water compliance monitoring devices (CMDs) have been developed. This includes various sensors, instruments, kits, methods, and assays that have been designed to assess compliance with ballast water discharge standards and requirements. Additionally, several novel CMD approaches are also currently being explored. However, rigorous, transparent and standardized verification testing is needed for these devices to be adopted and implemented globally, by multiple administrations (i.e. countries, governments, or jurisdictions) to enforce compliance monitoring. Otherwise, CMD performance, data quality, and uncertainties will remain unknown. To address this need, this protocol has been developed by a subgroup of the ICES/Intergovernmental Oceanographic Commission (IOC)/IMO Working Group on Ballast and other Ship Vectors (WGBOSV) to serve as a standardized framework for the verification testing of CMDs.
ballast water; non-native; compliance monitoring device; monitoring10.17895/ices.pub.5465WGBOSV
TextTamburri, M.N., Bailey, S.A., Everett, R.A., First, M.R., Gollasch, S., Outinen, O., and Drake, L.A.2707-6997978-87-7482-250-96/18/2020 7:45 PM
TIMES64.pdf
  
2021TimesICES Survey Protocols – Manual for acoustic surveys coordinated under ICES Working Group on Acoustic and Egg Surveys for Small Pelagic Fish (WGACEGG)EOSG
64
4/19/2021 10:58 AMRuth Anderson
This manual documents the methodologies used to collect and analyse acoustics data during surveys coordinated ICES Working Group on Acoustic and Egg Surveys for Small Pelagic Fish (WGACEGG). The group coordinates ten individual acoustic surveys conducted in the Northeast Atlantic shelf waters (ICES Areas 6, 7, 8 and 9) by five countries (Portugal, Spain, France, UK, and Ireland). These surveys account for about 240 at-sea days per year on average. The group coordinates joint surveys covering shelf waters from southern Portugal to Ireland in spring and autumn, and an individual survey in the Gulf of Cadiz in summer. The spring and autumn joint surveys are a combination of five acoustic surveys.
survey; survey data; Acoustic data; fish; stock assesment; fish biomass; acoustic survey10.17895/ices.pub.7462WGACEGG
TextDoray, M., Boyra, G., and van der Kooij, J. (Eds.)2707-6997978-87-7482-632-34/15/2021 2:36 PM