Optical methods

By K. Foote

 

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Contents

 

7.1 Introduction

7.2 General discussion of principles, techniques and comparative approaches

-         Review

-         Basic principles

-         Light phenomena

-         Illumination

-         Water as an optical medium

-         Light detection

-         Magnification and resolution

-         Sources of variability

-         Instrument effects

-         Water medium

-         Animal-dependent effects

-         Classes of light microscopy

-         Imaging

-         Bright – field microscopy

-         Contrast techniques

-         Fluorescence microscopy

-         Quantification

-         Techniques

-         Silhouette photography

-         Optical plankton counting

-         Video plankton recording

-         Comparisons

-         Identification

-         Imaging versus quantification

-         Venue and deployment

-         Operating range of two systems

7.3 Measurement protocols, model computations and examples

-         Silhouette photography in the laboratory

-         Procedures

-         Further processing of the film

-         Optical plankton counter

-         Calibration including comparisons

-         Limitations

-         Operating scenario

-         Applications

-         Video plankton recorder

-         Calibration including comparisons

-         Limitations

-         Operating scenario

-         Applications

7.4 Acknowledgements

7.5 Refrences

 

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