Methods for population genetic analysis of zooplankton By A. Bucklin

Contents Questions Comments and corrections New protocols Back to the main page

Contents 11.1 Background 11.2 Technical approaches to determining genetic diversity - Allozymes - Restriction fragment length polymorphisms of DNA - DNA sequence analysis - Oligonucleotide probe hybridization - Allele-specific PCR - Microsatellite DNA - RAPDs - New emerging techniques 11.3 Statistical approaches to assessing genetic diversity and structure - Statistical measures of genetic diversity - Statistical measures of genetic structure - Statistical analysis of gene flow (dispersal) - Computer methods and software sources 11.4 Strategies for preservation of zooplankton samples for genetic analysis - Preservation and storage in ethanol - Quick freezing in liquid nitrogen - Formalin, glutaraldehyde, and other bad things - Dehydration - In situ molecular analysis 11.5 General recommendations 11.6 Measurement protocols - Introduction - Facilities and equipment - General laboratory rules - Procedures - Sample preservation - DNA purification - PCR amplifications - Gel electrophoresis - Gel purification of DNA - Recipes and safety information - Buffers and frequently used solutions - Safety information 11.7 Further reading 11.8 Acknowledgements 11.9 References Top of the Document Questions Top of the Document Comments and corrections Top of the Document New Protocols Top of the Document